In vitro toxicity of Niedenzuella (Tetrapterys) multiglandulosa on bovine embryos

Abstract Niedenzuella (Tetrapterys) multiglandulosa, a vine plant found in Brazil, has been correlated to outbreaks of poisoning cattle and buffaloes, generating economic losses related the death due heart failure, miscarriage, abortion, stillbirth, neonatal mortality. The aim this study was examine embryotoxic potential aqueous extract on vitro bovine embryos. In performed five replicates embryo culture assigned two groups: control, medium without extract; treated group, with addition 2.7mg/mL (10%) sixth day culture. Cleavage rate evaluated at 2 cell Viability, hatchability underdevelopment blastocysts seventh, eighth, ninth days (D7, D8, D9, respectively) were assessed under stereoscopic microscope. On 7, submitted TUNEL assay determine apoptotic index. exposure embryos N. multiglandulosa resulted reduced development survival, by dark cytoplasm indicating poor morphology quality marked reduction hatchability. We observed significant blastocyst production/number cleaved (60.6% vs 41.5%); production/total number matured oocytes (35.1% 21.3%); embryonic hatching rates (38.0% 10.0%). However, no effects rate. conclusion, leaves reduces viability vitro, suggesting possible detrimental development.